Product Info Summary
SKU: | P00261 |
---|---|
Size: | 100 μl/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IP, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Phospho-NAK/TBK1 (S172) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00261
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Phospho-NAK/TBK1 (S172) Rabbit Monoclonal Antibody catalog # P00261. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-NAK/TBK1 (S172) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00261)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
23T28
Isotype
IgG
Immunogen
A synthesized peptide derived from human Phospho-NAK/TBK1 (S172)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00261 is reactive to TBK1 in Human, Mouse, Rat
Applications
P00261 is guaranteed for IP, WB Boster Guarantee
Observed Molecular Weight
84 kDa
Calculated molecular weight
83.642kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IP 1:50
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of NAK/TBK1 using anti-NAK/TBK1 antibody (P00261).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human U-87MG whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAK/TBK1 antigen affinity purified monoclonal antibody (Catalog # P00261) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NAK/TBK1 at approximately 84 kDa. The expected band size for NAK/TBK1 is at 84 kDa.
Protein Target Info & Infographic
Gene/Protein Information For TBK1 (Source: Uniprot.org, NCBI)
Gene Name
TBK1
Full Name
Serine/threonine-protein kinase TBK1
Weight
83.642kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11; EC 2.7.11.1; FLJ11330; FTDALS4; NAK; NAK serine; NAK serine/threonine-protein kinase TBK 1; NAK; NAKserine/threonine-protein kinase TBK1; NF-kappa-B-activating kinase; NFKB-Activating Kinase; T2K; TANK; TANK binding kinase; TANK-binding kinase 1NF-kB-activating kinase; TBK 1; TBK1 TBK1 FTDALS4, IIAE8, NAK, T2K TANK binding kinase 1 serine/threonine-protein kinase TBK1|NF-kB-activating kinase|NF-kappa-B-activating kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TBK1, check out the TBK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TBK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-NAK/TBK1 (S172) Rabbit Monoclonal Antibody (P00261)
Hello CJ!
No publications found for P00261
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Phospho-NAK/TBK1 (S172) Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-Phospho-NAK/TBK1 (S172) Rabbit Monoclonal Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question